Per: WENDELL BRUNO ALMEIDA BEZERRA (IME), Ulisses oliveira costa (ime), suzana barreto noronha ribeiro (ime), michelle oliveira costa (ime), fernanda santos da luz (ime), marcelo henrique prado da silva (ime), sergio neves monteiro (ime)
Abstract:
In past years, the need for innovative products aimed at replacing, correcting and improving poorly performing tissues in humans or animals has increased with the development of technology. Considering this, the collagen stands out given its positive characteristics in its applications in tissue engineering. Fish collagen (FC) is found to be an interesting alternative source of this material. Thus, the objective of this work was to study the influence of the chemical treatment of pirarucu scales in acid solution, aiming to remove the present hydroxyapatite nanocrystals, in order to obtain pure collagen. To this end, a dilute acid solution of HCl (0.6N) was prepared, and the scales were immersed for 5 days. SEM and XRD analyzes were used to verify the treatment efficiency. Through the obtained electromicrographs, one could verify the alteration of the surface morphology of the scales, while the diffractograms confirmed the presence of the peaks related to the hydroxyapatite in the in natura scales and the absence of these in the treated scales, proving the partial or total removal of that phase.